|Alternative algorithm for poliovirus isolation and characterization|
As the countries of the world advance towards the eradication of wild poliovirus, it is necessary to maintain good quality laboratory results and improve the timeliness of polio confirmation.
To achieve this, the Global Polio Laboratory Network (GPLN) of the World Health Organization (WHO) recommended the implementation of a new algorithm for viral isolation, using the cells RD and L2OB simultaneously, permitting the report of results within 14 days of receipt of the stool specimen in the laboratory (previously, 28 days were considered appropriate).
PAHO continues to be committed to the global eradication of polio; therefore it will monitor the implementation of this algorithm, and update its Weekly Polio Bulletin in order to show compliance with laboratory indicators.
For more information, please refer to the "Supplement to the WHO Polio Laboratory Manual" available below: http://www.who.int/immunization_monitoring/laboratory_polio_resources/en/index.html
Regional Office for the Americas of the World Health Organization