Real-time RT-PCR (TaqMan™) protocol – Yellow fever virus (YFV) 17D vaccine strain

This document provides detailed instructions for conducting real-time RT-PCR assays to detect the yellow fever virus, specifically the YFV 17D vaccine strain. It includes information on the preparation of the master mix, cycling conditions, and interpretation of results. The protocol is designed to ensure accurate and reliable detection of the virus in various samples.